Lung Cancer SPORE

Publications

Recent Publications

Wilson DO, Weissfeld JL, Fuhrman CR, Fisher SN, Balogh P, Landreneau RJ, Luketich JD, Siegfried JM. The Pittsburgh Lung Screening Study (PLuSS): outcomes within 3 years of a first computed tomography scan. Am J Respir Crit Care Med. 2008 Nov 1;178(9):956-61 PMID 18635890.

Wilson DO, Weissfeld JL, Balkan A, Schragin JG, Fuhrman CR, Fisher SN, Wilson J, Leader JK, Siegfried JM, Shapiro SD, Sciurba FC. Association of Radiography Emphysema and Airflow Obstruction with Lung Cancer. Am J. Respir Crit Care Med 2008 178(7):738-44. PMID 18565949.

Dubinett SM, Aberle DR, Tashkin DP, Mao JT. The Partners—Airflow Obstruction, Emphysema, and Lung Cancer. Am J Respir Crit Care Med, 2008 Oct 1;178(7):665-6 (Editorial on Wilson DO article) PMID 18796652.

Lustgarten, J.L., Gopalakrishanan, V., Malehorn, D., Bigbee, W. Assigning Putative Protein Identifications to Selected Lung Cancer Biomarkers from Surface-Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of Blood Serum. Presented at the Annual Advancing Pathology Imaging, Informatics and the Internet (APIII) conference (2008). Honorable Mention Award.

Jill M. Siegfried, Laura P. Stabile, Pamela A. Hershberger, Edward Garon, Richard Pietras, Anne M. Traynor, and Joan H. Schiller. Role of Estrogen and Estrogen Receptors in Growth and Progression of Non-Small Cell Lung Cancer. Presented at the NCI Translational Science Meeting, Washington, DC, Nov. 7-9, 2008.

Joel S. Weissfeld, David O. Wilson, Carl R. Fuhrman, Stephen N. Fisher, Rodney J. Landreneau, James D. Luketich, and Jill M. Siegfried. The Pittsburgh Lung Screening Study (PLuSS): A Research Cohort of Current and Former Cigarette Smokers Screened for Lung Cancer with Helical Computerized Tomography (CT). Presented at the NCI Translational Science Meeting, Washington, DC, Nov. 7-9, 2008.

Project 1

Zhang, Q., Thomas, S.M., Lui, V.W.Y., Xi, S., Siegfried, J.M., Fan, H., Smithgall, T., Mills, G., Grandis, J.R. Phosphorylation of TNF- converting enzyme by Gastrin-releasing peptide induces amphiregulin release and EGFR receptor activation. Proc. National Acad. Science. 103: 6901-6906, 2006.

Carlisle, D., Xuwan, L., Hopkins, T.M., Swick, MC., Dhir, R., Siegfried, J.M. Nicotine Activates Cell Signaling Pathways through Muscle-type and Neuronal nicotinic Acetylcholine Receptors in Non-small Cell Lung Cancer Cells. Pulmonary Pharmacology and Therapeutics doi:10.1016/j.pupt.2006.07.001.

Kelloff GJ, Lippman SM, Dannenberg AJ, Sigman CC, Pearce HL, Reid BJ, Szabo E, Jordan VC, Spitz MR, Mills GB, Papadimitrakopoulou VA, Lotan R, Aggarwal BB, Bresalier RS, Kim J, Arun B, Lu KH, Thomas ME, Rhodes HE, Brewer MA, Follen M, Shin DM, Parnes HL, Siegfried JM, Evans AA, Blot WJ, Chow WH, Blount PL, Maley CC, Want KK, Lam S, Lee JJ, Dubinett SM, Engstrom PF, Meyskens FL Jr., O’Shaughnessy J, Hawk ET, Levin B, Nelson WG, Hong WK. AACR Task Force on Cancer Prevention. Progress in Chemoprevention Drug Development: the Promise of Molecular Biomarkers for Prevention of Intraepithelial Neoplasia and Cancera Plan to Move Forward. Clinical Cancer Research 12: 3661-97, 2006.

Zhang, W, Stabile, LP, Keohavong, P, Romkes, M, Grandis, JR, Traynor, AM, Siegfried, JM. Mutation and Polymorphism in the EGFR-TK Domain Associated with Lung Cancer. Journal of Thoracic Oncology 7:635-647, 2006.

Zhang, W, Z, Weissfeld, JL, Romkes, J, Land, SR, Grandis, JR, Siegfried, JM.. Association of the EGFR Intron 1 CA Repeat Length with Lung Cancer Risk. In Press, Molecular Carcinogenesis.

Myerberg, M.M., Latoche, J.D., McKenna, E.E., Stabile, L.P., Siegfried, J.M., Feghali-Bostwick, C.A., Pilewski, J.M. HGF and other fibroblast secretions modulate the phenotype of human bronchial epithelial cells. In press, Am. J. Physiol. Lung, 2007.

Liu, X, Carlisle, DL, Swick, MC, Gaither Davis, A, Grandis, JR, Siegfried, JM. Gastrin-releasing peptide activates Akt through the epidermal growth factor receptor pathway and abrogates the effects of gefitinib. In press, Experimental Cell Research, 2007.

Project 2

Saito H, Dubsky P, Dantin C, Finn OJ, Banchereau J, Palucka AK. Cross-priming of cyclin B1, MUC-1 and survivin specific CD8+T cells by dendritic cells loaded with killed allogeneic breast cancer cells. Breast Cancer Res. 2006 Nov 27;8(6):R65

Vella, L.A., Finn, O.J., "Innate and Adaptive Immunity in Lung Cancer: The Balance between Cancer Progression and Prevention." In Novartis Found Symp. 2006;279:206-12 (book chapter)

Vella, LA. and Finn OJ. "Humoral and Cellular Response to the Self(tumor)-antigen Cyclin B1 in Lung Cancer Patients and Healthy Donors" AACR Special Conference in Tumor Immunology, Nov 29-Dec 4, 2006, Miami, FL. Vella, LA, Yu, M, Siegfried, JM, Finn, OJ. Cyclin B1 and Lung Cancer: Significance of the Immune Response. 14th Annual SPORE Investigators’ Workshop, July 2006.

Project 3

Abstracts

Intersession Reproducibility and Independent Clinical Cohort Evaluation of Lung Cancer Serum Proteomic Profiling and Classification Using SELDI-TOF-MS. William L. Bigbee, David E. Malehorn, Talal El-Hefnawy, Milos Hauskrecht, James Lyons-Weiler, Richard C. Pelikan, Mai Sun, Rodney J. Landreneau, James D. Luketich, Joel L. Weissfeld, Jill M. Siegfried, and Pierre P. Massion. Presented at the Lung SPOREs Midyear Meeting, Los Angeles, CA, January 25-26, 2006. Proteomic Signature of Lung Cancer from Analysis of Unfractionated Serum. Yildiz PB, Shyr Y, Rahman SMJ, Wardwell NR, Zimmerman LJ, Shakhtour B, Gray WH, Shuo Chen, Moore JH, Liebler DC, Bigbee WL, Siegfried JM, Weissfeld JL, Gonzalez AL, Ninan M, Johnson DH, Carbone DP, Caprioli RM, and Massion PP. Presented at the Lung SPOREs Midyear Meeting, Los Angeles, CA, January 25-26, 2006. Metabolomic Biomarkers of Non-small Cell Lung Cancer. Thomas J. Colatsky, Judong Shen, Alan J. Higgins, Imran Shah, Talal El-Hefnawy, Jill M. Siegfried, and William L. Bigbee. Presented at the 14th SPORE Investigators’ Workshop, Baltimore, MD, July 15-19, 2006.

Multiplexing Approach for Early Diagnosis of Lung Cancer. A. Lokshin, A. Lisovich, B. Nolen, A. Marrangoni, L. Velikokhatnaya, M. Winans, B. Bigbee, J. Siegfried, J. Weissfeld, T. El-Hefnawy, and A. Lokshin. Presented at the 14th SPORE Investigators’ Workshop, Baltimore, MD, July 15-19, 2006.

Publications

Chen G, Wang X, Yu J, Varambally S, Yu J, Thomas DG, Lin M-Y, Vishnu P, Wang Z, Wang R, Fielhauer J, Ghosh D, Giordano TJ, Giacherio D, Chang AC, Orringer MB, El-Hefnawy T, Bigbee WL, Beer DG, Chinnaiyan AM (2007) Autoantibody profiles reveal ubiquilin 1 as a humoral immune response target in lung adenocarcinoma. Cancer Res (in press).

Xi L. Coello MC. Litle VR. Raja S. Gooding WE. Yousem SA. El-Hefnawy T. Landreneau RJ. Luketich JD. Godfrey TE. A combination of molecular markers accurately detects lymph node metastasis in non-small cell lung cancer patients. Clinical Cancer Research. 12(8):2484-91, 2006

Liu Y. Lan Q. Siegfried JM. Luketich JD. Keohavong P. Aberrant promoter methylation of p16 and MGMT genes in lung tumors from smoking and never-smoking lung cancer patients. Neoplasia 8(1):46-51, 2006

Xi L, Nicastri DG, El-Hefnawy T, Hughes SJ, Luketich JD, Godfrey TE. Optimal markers for real-time quantitative reverse transcription-PCR detection of circulating tumor cells from melanoma, breast, colon, esophageal,head and neck, and lung cancers. Clin Chem, In Press

Accepted for Presentation

Pennathur A, Burton SA, Luketich JD, Abbas G, Chen M, Heron DE, Gooding WE, Ozhasoglu C, Landreneau RJ, Christie NA. Stereotactic Radiosurgery for the Treatment of Stage I Non-small Cell Lung Cancer in High-Risk Patients. American Association of Thoracic Surgery Washington, DC. May 5-7, 2007.

Litle VR, Feber A, Xi L, Luketich JD, Pennathur A, Landreneau RJ, Wu M, Swanson SJ, Godfrey TE. MicroRNA Expression Profiles in Esophageal Cancer. American Association of Thoracic Surgery Washington, DC. May 5-7, 2007.

d’Amato TA, Fernando HC, Chan BY, Ricketts WA, Luketich JD, Landreneau RJ. Differential gene expression associated with early tumor recurrence and long disease free survival in early stage lung cancer. Society of Surgical Oncology Washington, D.C. March 15-18, 2007

Schuchert MJ, d’Amato T, Landreneau RJ. Radiation exposure to treatment team from intraoperative 125I Brachytherapy Implants as Adjuvant Therapy to Sublobar Resection in Stage I Lung Cancer. Society of Surgical Oncology Washington, D.C. March 15-18, 2007

Shamji FM, d’Amato TA, Ashrafi AS, Maziak DE, Sundaresan SR, Al-Shehlb D, El-Sherif A, Ferson PF, Luketich JD, Landreneau RJ. Should pneumonectomy be avoided in patients with locally advanced non-small cell lung cancer following induction chemotherapy? Society of Surgical Oncology Washington, D.C. March 15-18, 2007

Project 4

Publications

Gao WM, Romkes M, Siegfried JM, Luketich JD, Keohavong P. Polymorphisms in DNA repair genes XPD and XRCC1 and p53 mutations in lung carcinomas of never-smokers. Mol Carcinogenesis. 2006;45: 828-32.

Zhang W, Stabile LP, Keohavong P, Romkes M, Grandis JR, SchillerJH, Traynor AM, Siegfried JM. Mutation and Polymorphism in the EGFR-TK Domain Associated with Lung Cancer. J Thor Oncol. 2006;1:635-47. (see Project 1)

Raimondi S, Paracchini V, Autrup H, Benhamou S, Boffetta P, Cote ML, Dialyna IA, Dolzan V, Dragani T, Garte S, Hirvonen A, Husgafvel-Pursiainen K, Imyanitov EN, Kalina I, Kang D, Kiyohara C, Kremers P, Lan Q, Neri M, Povey AC, Rannug A, Reszka E, Risch A, Romkes M, Ruano-Ravina A, Schneider J, Seow A, Shields PG, Sobti RC, Srensen M, Spitz MR, Strange RC, Stcker I, Sugimura H, To-Figueras J, Tokudome S, Yang P, Yokota J, Warholm M, Taioli E. Pooled and meta analysis of GSTT1 polymorphism and lung cancer: a HuGE-GSEC review. Am J Epidemiol. 2006;164:1027-42.

Zhang W, Weissfeld JL, Romkes M, Land S, Siegfried JM. Association of the EGFR Intron 1 CA Repeat Length with Lung Cancer Risk. Mol Carcinogenesis. 2006, accepted (see Project 1)

Abstracts

Romkes M, Buch S, Taron M, Rosel R. Evaluation of Nucleotide Excision Repair (NER) gene pathway haplotypes with platinum drug resistance and survival in NSCLC. SPECS annual meeting, 2006;2:12.

Styn MA, Perkins KA, Land SR, Romkes M, Weissfeld JL. Does an abnormal CT finding predict smoking cessation one year later? Society of Behavioral Medicine Annual Meeting, March 2007, accepted.

Administrative Core

Announcement of Lung Cancer Awareness Month event on November 29, 2006

Reprint of Letter to the Editor from Dr. Jack Hill to the Woodland Progress (NJ)

Reprint of Letter to the Editor from Dr. Jack Hill to the Pittsburgh Post-Gazette

Text of testimony given by Dr. Jack Hill to the Pennsylvania State Senate Select Committee on Tobacco Settlement dated September 26, 2006

Reprint of article Women Lift Smokescreen on Lung Cancer published in Bay Area Business Woman (CA) in November of 2005

Reprint of article The Thinkers: Celebrity Deaths Shine Light on Lung Cancer published in the Pittsburgh Post-Gazette on March 27, 2006

UPMC Cancer Centers and University of Pittsburgh Cancer Institute capital campaign brochure feature Dr. Jill M. Siegfried, Lung SPORE PI, Dr. William L. Bigbee, Lung SPORE Basic science Associate Director, and Dr. James D. Luketich, Lung SPORE Clinical Associate Director

Tissue and Blood Bank Core

Carlisle, D., Xuwan, L., Hopkins, T.M., Swick, MC., Dhir, R., Siegfried, J.M. Nicotine Activates Cell Signaling Pathways through Muscle-type and Neuronal nicotinic Acetylcholine Receptors in Non-small Cell Lung Cancer Cells. Pulmonary Pharmacology and Therapeutics doi:10.1016/j.pupt.2006.07.001. (see Project 1)

Stabile, L.P., Lyker, J.S., Land, S.R., Dacic, Sanja, Zamboni, BA, Siegfried, J.M. Transgenic mice overexpressing hepatocyte growth factor in the airways show increased susceptibility to lung cancer. Carcinogenesis 27: 1547-55, 2006.

Biostatistics/Bioinformatics Core

S. Land, A.M. Egloff, J. Weissfeld, and O. Finn, “Statistical Estimation of Normal Ranges for Serum Anti-Cyclin B1 Antibodies in a Population at High Risk for Lung Cancer”, Winter Lung SPORE meeting, Los Angeles, CA, January 2006.

M.A. Styn, K.A. Perkins, S.R. Land, M. Romkes, J.L. Weissfeld "Does an abnormal CT finding predict smoking cessation one year later?" Society of Behavioral Medicine's 28th Annual Meeting and Scientific Sessions, March 21-24, 2007, Washington, DC (see Project 4)

Egloff AM, Weissfeld J, Land SR, Finn OJ. Evaluation of anticyclin b1 serum antibody as a diagnostic and prognostic biomarker for lung cancer. Ann N Y Acad Sci. 2005 Dec;1062:29-40. PMID: 16461786

Stabile LP, Lyker JS, Land SR, Dacic S, Zamboni BA, Siegfried JM. Transgenic mice overexpressing hepatocyte growth factor in the airways show increased susceptibility to lung cancer. Carcinogenesis 27(8):1547-55, 2006, PMID: 16513678.

Stabile LP, Rothstein ME, Kim KJ, Land SR, Luong TM, Siegfried JM. Anti-HGF monoclonal antibody therapy decreases incidence of lung tumors in transgenic mice overexpressing HGF. AACR, 2007. (see Tissue and Blood Bank Core)

Day, R. Joint Statistical Meetings, July 29-August 2, Salt Lake City, Utah, 2007.

Developmental Research Program

Xi L, Lyons-Weiler J, Coello MC, Huang X, Gooding WE, Luketich JD, Godfrey TE. Prediction of Lynph Node Metastasis by Analysis of Gene Expression Profiles in Primary Lung Adenocarcinomas. Clin Cancer Res 2005;11(11) 2005

Xi, L, Coello MC, Litle, VR, Raja S, Gooding WE, Yousem SA, El-Hefnawy T, Landreneau R, Luketich JD, Godfrey TE. A Comination of Molecular Markers Accurately Detects Lymph Node Metastasis in Non-SmallCell Lung Cancer Patients. Clin Cancer Res 2006;12(8), 2006. (see Project 3)

Achiwa H, Lazo JS. “The Role of Cdc25B in Lung Carcinogenesis” Cancer Res 2007; 67(2), 2007.

Styn MA, Perkins KA, Land SR, Romkes M, Weissfeld JL. Does an abnormal CT finding predict smoking cessation one year later? Society of Behavioral Medicine Annual Meeting, March 2007, accepted. (see Project 4)

Career Development Program

Pamela Hershberger, PhD:

Publications

Parise PA, Egorin MJ, Kanterewicz b, Taimi M, Petkovich M, Lew AM, Chuang SS, Nichols M, El-Hefmawy T, Hershberger PA. CYP24, the enzyme that catabolizes the antiproliferative agent vitamin D, is increased in lung cancer. Int J Cancer 119, 1819-1828, 2006.

Abstracts

Kanterewicz B, Nichols m, Hershberger PA. Elucidation of the mechanisms that control expression of the vitamin D metabolizing enzyme, CYP2, in lung cancer cells. Presented at the 14th SPORE Investigators’ Workshop, Baltimore, MD, July 15-19, 2006.

Laura Stable, PhD:

Publications

Stabile, L.P., Lyker, J.S., Land, S.R., Dacic, Sanja, Zamboni, BA, Siegfried, J.M. Transgenic mice overexpressing hepatocyte growth factor in the airways show increased susceptibility to lung cancer. Carcinogenesis 27: 1547-55, 2006 (see Biostatistics/Bioinformatics Core)

Stabile, L.P., Lyker, J.S., Gubish, C.T., Zhang, W., Grandis, J.R., Siegfried, J.M. Combined Targeting of the Estrogen Receptor and the Epidermal Growth Factor Receptor in Non-Small Cell Lung Cancer Shows Enhanced Anti-Proliferative Effects. Cancer Research 65 : 1459-1470, 2005.

Abstracts

Stabile LP, Rothstein ME, Kim KJ, Land SR, Luong TM, Siegfried JM. Anti-HGF monoclonal antibody therapy decreases incidence of lung tumors in transgenic mice overexpressing HGF. AACR, 2007. (see Biostatics/Bioinformatics Core)

Stabile LP, Paprzycki C, Grandis JR, Siegfried JM. Cross-talk between the estrogen receptor and the epidermal growth factor receptor in head and neck squamous cell carcinoma. Proceedings of the American Association for Cancer Research. Volume 47, April 2006. Washington, DC.

Stabile LP, Lyker JS, Barrow ME, Gubish CT, Siegfried JM. Non-nuclear estrogen signaling mechanisms in NSCLC. Flight Attendant Medical Research Institute 5th Scientific Symposium. May 2006. Cambridge, MA.

Stabile LP, Lyker JS, Gubish CT, Zhang W, Grandis JR, Siegfried JM. Combined targeting of the estrogen receptor and the epidermal growth factor receptor for lung cancer therapy. 17th Annual UPCI Scientific Retreat. June 2005. Pittsburgh, PA.

Siegfried JM, Stabile LP, Liu X, Thomas SM, Grandis JR. Novel pathways for lung cancer therapy. AACR special conference. Molecular Pathogenesis of Lung Cancer: Opportunities for Translation to the Clinic. Feb 2005. San Diego, CA.

Siegfried JM, Stabile LP, Hershberger PA, Lyker JS, Gubish CT, Vasquez C, Nichols MD. Targeting the estrogen receptor and the epidermal growth factor receptor for lung cancer therapy. Annual National Cancer Institute SPORE Investigators' Meeting. July 2004. Baltimore, MD.

Stabile LP, Lyker JS, Gubish CT, Siegfried JM. Targeting the estrogen receptor and the epidermal growth factor receptor for lung cancer therapy. Proceedings of the American Association for Cancer Research. Volume 45, March 2004. Orlando, FL.

Stabile LP, Lyker JS, Gubish CT, Siegfried JM. Targeting the estrogen receptor and epidermal growth factor receptor pathways for lung cancer therapy. Annual Flight Attendant Medical Research Institute Symposium. May 2004. Miami, FL.

El-Hefnawy T, Stabile LA, Siegfried JM, Donovan MA, Davis DL, Luketich JD. Endocrine Disrupting Compounds Can Regulate Lung and Breast Cancer Proliferation, Migration and Response to Chemotherapy. 17th Annual UPCI Scientific Retreat. June 2005. Pittsburgh, PA.

Lin Zhang, PhD:

Publications

Yu, J., Yue, W., Wu, B. and Zhang, L. (2006) PUMA sensitizes lung cancer cells to chemotherapeutic agents and irradiation. Clinical Cancer Research 12: 2928-2936.

Ding, W.X., Ni, H.M., Chen, X.Y., Yu, J., Zhang, L., and Yin, X.M. (2007) A coordinated action of Bax, PUMA and p53 promotes MG132-induced mitochondria activation and apoptosis in colon cancer cells. Molecular Cancer Therapeutics (In Press).

Wang, P., Yu, J. and Zhang, L. (2007) The nuclear function of p53 is required for PUMA-mediated apoptosis induced by DNA damage. Proc. Natl. Acad. Sci. USA. (In Press).

Yu, J., Wang, P., Ming, L.H., Wood, M. and Zhang, L. (2007) SMAC/Diablo mediates the proapoptotic function of PUMA by regulating PUMA-induced mitochondrial events. Oncogene [Epub ahead of print] Jan 22.

Wu, B., Qiu, W., Wang, P., Yue, W., Yu, H., Cheng, T., Zambetti G.P., and Zhang, L.* and Yu, J.* (2006) p53-independent induction of PUMA mediates intestinal apoptosis induced by ischemia reperfusion. Gut [Epub ahead of print] Nov 24 (* corresponding authors).

Ming, L.H, Wang, P., Bank, A., Yu, J., and Zhang, L. (2006) PUMA dissociates Bax from Bcl-XL to induce apoptosis in colon cancer cells. Journal of Biological Chemistry 281:16034-16042.

Wang, H.J., Qian, H.L., Yu, J., Zhang, XY, Zhang, L., Fu, M, Liang, X, Zhan, Q.M. and Chen, L. (2006) Administration of PUMA adenovirus increases the sensitivity of esophageal cancer cells to anticancer drugs. Cancer Biology & Therapy. 5:380-385.

Jiang, M., Wei, Q., Du, Q., Yu, J., Zhang, L. and Dong, Z. (2006) Regulation of PUMA by p53 in cisplatin-induced renal cell apoptosis. Oncogene 25:4056-4066. Yu, J. and Zhang, L. (2005) The transcriptional targets of p53 in Apoptosis Control. Biochem. Biophys. Res. Comm. 331:851-858.

Abstracts

Yue, W., Guo, M.Z., Zhang, W.P., Jill Siegfried, J.M., Yu, J., Zhang, L. Identification of novel methylation markers of lung cancer. Proc Amer Assoc Cancer Res 2006; 47: [Abstract #48].

Yu, J., Yue, W., Wu, B., and Zhang, L., PUMA Sensitizes Lung Cancer Cells to Chemotherapeutic Agents and Irradiation. Proc Amer Assoc Cancer Res 2006; 47: [Abstract #2209].