Lung Cancer SPORE

Projects

PROJECT 3: SERUM PROTEOMIC BIOMARKERS FOR LUNG CANCER DETECTION AND PROGNOSIS
PROGRESS REPORT

Co-Project Leaders: William L. Bigbee, PhD
James D. Luketich, MD

Overview

Our ongoing effort in the second year of the new Project 3 has been focused in six areas: 1) the final analysis of the preliminary Luminex and SELDI-TOF-MS data and manuscript preparation; 2) database development and clinical annotation of the SPORE Lung Cancer Registry case/control patient cohort and standardized blood sample collection resources; 3) optimization and refinement of the initial Luminex multianalyte serum panels (xMAP) to include new candidate lung cancer markers and independent verification of preliminary results on the Searchlight multiplexed immunoassay platform; 4) development and finalization of new workflows for the depletion and fractionation of plasma/serum specimens for MALDI-T0F-MS comparative proteomic profiling and, most recently, LC-MS based label-free peptide quantitation and protein identification profiling for biomarker discovery; 5) final study design and case/control sample pooling strategy for analysis using the parallel MS discovery workflows; and 6) development and funding of complementary collaborative biomarker studies.