Equipment
Automated Synthesizers:
Applied Biosystems ABI 433A Automated Peptide Synthesizer – used for routine peptide synthesis and for hydrophobic peptides due to conditional double coupling features; wide range of synthesis scales from 10 uM to 1.0 mM.
Applied Biosystems Pioneer Multiple Peptide Synthesizer – used for simultaneous production of two peptides on scale of 0.2 or 0.5 mM.
AAPPTEC Omega 396 Multiple Peptide Synthesizer – used for multiple peptide synthesis (40 or 96 reactor platforms) using 0.1 mM scale or 25 uM scale, respectively.
Applied Biosystem ABI 433A Peptide Synthesizer – used exclusively for Certified peptide production on a scale of 0.2 mM to 10 mM.
HPLC Peptide Purification Systems:
Waters Alliance Delta Prep System and Millennium 32 Software Package - general analytical and preparative peptide purifications
Waters Alliance with Delta Prep System and Empower Software Package - general analytical and preparative peptide purifications
Waters 486/600E System - general analytical and preparative peptide purifications
Waters 490E/600E System - general analytical and preparative peptide purifications
Waters LC3000 System - general analytical and preparative peptide purifications
Waters 486/600E System with Millennium 32 Software Package - analytical and preparative purifications of Certified peptides only
The Facility maintains a stock of diverse reverse phase analytical and preparative HPLC columns that range in capacity from microgram to 500 mg quantity peptide per run. These latter large capacity columns are used extensively for UPCI research applications.