News

December 2007

The UPCI Flow Cytometry Facility will observe the University of Pittsburgh's winter recess. The Facility will be closed December 24th and will reopen January 2nd. There will be no cell sorting or staff assisted appointments. Power users can arrange to use one of the User dedicated cytometers (Red or Green XLs) by contacting the staff before December 21st.

Look for new and exciting developments in 2008. The Facility will be expanding the availability (hours) of many services. We hope to expand on existing instrumentation and add a few developing technologies.

Happy Holidays

November 2007

Cassandra (Cassie) Singer, presently with Guava Technologies, (http://www.guavatechnologies.com) has placed the Guava EasyCyte benchtop flow cytometer in the UPCI Flow Facility. The EasyCyte is an easy-to-use flow cytometer that can sample from 96 well plates, as well as microcentrifuge tubes. Due to its unique capillary design, the EasyCyte is especially handy for assays involving very small sample volume. In addition, Guava supports applications with reagent kits and analytical software that make assays simple and affordable.

The EasyCyte is here on an extended loan in effort to explore the variety of uses that this technology offers. The facility is currently seeking groups that may be interested in developing assays that can be run on the EasyCyte. Please contact Michael Meyer (meyere@upmc.edu) or Cassie Singer (csinger@guavatechnologies.com) for additional information or an appointment to run the Guava EasyCyte.

October 2007

The UPCI Flow Cytometry Facility has been promoting the use of the Cyan multi-parameter flow cytometer for everday use. With the Cyan, one can take advantage of up to three lasers (405nm, 488nm, and 635nm) for excitation and can detect up to nine colors at one time. The staff can assist with running experiments; in addition, users with flow experience can be trained to run the Cyan on their own.

Please contact the staff if you have questions or are interested in learning more about using the Cyan.



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