Equipment
Major Analytical Chemical Instrumentation:
LC/MS System #1: Thermo Finnigan aQa mass spectrometer, Agilent 1100 autosampler, Agilent 1100 quaternary pump and degasser, operated under Thermo Finnigan Excalibur software.
LC/MS System #2: Agilent 1100 binary pump, Agilent 1100 autosampler, operated under Thermo Finnigan Excalibur software.
LC/MS System #3: Thermo Fisher MSQ single quadrupole mass spectrometer and HPLC system.
LC/MS/MS System #1: Waters Alliance 2695 HPLC module, Waters QuattroMicro triple stage mass spectrometer, operated under Masslynx version 4.0.
LC/MS/MS System #2: ABI 4000Q linear quadrupole tandem mass spectrometer.
HPLC System #1: Hewlett-Packard 1100 autosampler, Waters 501 and 510 pumps, a Waters 680 controller, a Waters model 2487 absorbance detector, and ChromPerfect data collection software.
HPLC System #2: Waters 717 autosampler with heater/cooler option, Waters 600 quaternary solvent delivery system with column oven, Waters 600E system controller, Waters 2487 programmable multiwavelength detector, Waters fraction collector, and a connection to.
HPLC System #3: Hewlett-Packard 1100 autosampler, Waters 6000 pump, Spectroflow 783 absorbance detector, and connection to ChromPerfect data collection software.
HPLC System #4: Hewlett Packard 1100 autosampler, Waters 501 pump, Spectroflow 757 absorbance detector, and a connection to ChromPerfect data collection software.
HPLC System #5: Hewlett Packard 1100 autosampler and 1050 quaternary pump with degasser, Waters 474 scanning fluorescence detector.
HPLC System #6: Hewlett Packard 1100 autosampler, Waters 501 pump, Hewlett Packard 1050 photodiode array detector, and Agilent Chemstation chromatography software.
HPLC System #7: Beckman System Gold 508 autosampler, Beckman System Gold 166 detector, Beckman System Gold 126 solvent module, IN/US beta-ram model 2B flow-through radio-isotope detector.
Additional HPLC Detectors and Equipment:
Bioanalytical Systems LC-4B electrochemical detector, Aminco fluoromonitor, Waters 660 gradient controllers (4), 680 gradient controller, Waters 440 absorbance detectors (5), Waters m 45 pump, Waters 6000 pump (3), Waters 501 pumps (3), Waters 510 pump, Alltech evaporative light scattering detector, ESA Coulochem model 5100A electrochemical detector, ChromPerfects chromatography software.
Other Analytical Chemical Instrumentation:
Perkin-Elmer 1100 atomic absorption spectrometer with EGA 400
graphite furnace, AS 40 furnace autosampler, data management software,
Hewlett-Packard Vectra computer, and Panasonic KX-P1091i printer. The
Facility is well equipped with state-of-the-art spectrophotometers;
fluorometers; ELISA readers; balances; centrifuges; microcomputers and
software; refrigeration, freezers, and ultracold freezers; chemical
fume hoods; incubators; and electrophoresis instrumentation.
HPLC instrumentation is operated through a unified data collection and analysis system, which is currently based on ChromPerfect Chromatography software. Each chromatograph is equipped with an interface that sends the collected raw data to a Gateway Pentium computer. Data collected by the computer can be archived and analyzed on site or remotely using analyze-only software. Data manipulation includes reintegration, recalculation, baseline subtraction, run comparison, ratioing, and batch reprocessing of the data. Data can be transferred via the closedcircuit cable network linking the individual PCs within the UPCI laboratories. Data may be graphically displayed in a number of formats.
Suitable facilities exist for handling irritating, toxic, and potentially hazardous materials. There are ample mical fume hoods and numerous biohazard laminar air flow hoods. As a requirement of our having served previously as the Pharmacology and Therapeutic Drug Monitoring Center for the Veterans Administration AIDS Treatment Cooperative Study, the Facility has established procedures and an isolated facility for the handling of infectious samples.