University of Pittsburgh Cancer Institute (UPCI)

Images

Catalog of Images from the UPCI Cell Culture and Cytogenetics Facility

The fluorescence in situ hybridization images, comparative genomic hybridization analyses and images, and the karyotype images were generated on the CytoVision Ultra System from Applied Imaging, Santa Clara, CA.

• Image 1: FISH
  FHIT exons 5 (green) and 9/10 (red) hybridized to control lymphocyte metaphase. Images 1-3 are derived from the research of Susanne Gollin, Kay Huebner, and Carlo Croce, funded by NIH DE10513, CA39860, and CA21124.
• Image 2: FISH
  FHIT exons 5 and 9/10 hybridized to UPCI:SCC084 interphase nuclei. Note absence of one copy of each exon in this oral carcinoma cell line.
• Image 3: FISH
  FHIT exons 5 and 9/10 hybridized to a UPCI:SCC056 interphase nucleus. Note absence of both copies of exon 5 and one copy of exons 9/10 in this oral carcinoma cell line.
• Image 4: FISH
  INT2 (red; 11q13) and D11Z1 (green; chromosome 11 centromere) probes hybridized to UPCI:SCC131 metaphase. Note amplification of INT2 in this oral carcinoma cell line. Probes from Oncor, Inc., Gaithersburg, MD. Results from the research of Susanne Gollin, funded by NIH DE10513.
• Image 5: Two representative G-banded metaphases from a mouse leukemia cell line.
• Image 6: Abnormal NHP karyotype.
• Image 7: Breast cancer cell line karyotype Representative abnormal karyotype of a human breast cancer cell line studied by Dr. Jean Latimer.
• Image 8: Paraffin section interphase FISH.
  Amplification of the cyclin D1 gene (CCND1) shown by fluorescence in situ hybridization (FISH) on a paraffin section of a head and neck squamous cell carcinoma.
• Image 9: Metaphase FISH
  Amplification of the cyclin D1 gene (CCND1) shown by fluorescence in situ hybridization on metaphase chromosomes from a head and neck squamous cell carcinoma cell line.
• Image 10: Near-tetraploid murine karyotype.
  Near-tetraploid murine chromosome complement.
• Image 11: VNTR FISH
  Identification of normal and aberrant chromosomes 15 in a CHO hybrid metaphase cell studied by Dr. Robert Nicholls.