University of Pittsburgh Cancer Institute (UPCI)

Overview

The Cell Culture and Cytogenetics Facility provides critical cell culture services including cell line authentication, Mycoplasma testing, primary cell culture of mammalian cells, and Epstein-Barr virus (EBV) transformation of human B-lymphocytes, as well as comprehensive, state-of-the-art cytogenetics services including assisting investigators with study design, performing cell culture, preparing probes, carrying out and interpreting classical and molecular cytogenetic analyses, and assisting in manuscript preparation for UPCI and University of Pittsburgh investigators.

Classical and molecular cytogenetic analyses include karyotyping, special staining and banding procedures, chromosomal instability assessment, and fluorescence in situ hybridization (FISH) that can be performed on cells from blood or tissue specimens from humans or experimental animals, cultured cells, and/or fresh, frozen, or paraffin-embedded tumor biopsies, and enable investigators to:

  • characterize the karyotype of cells;
  • map the location of new genes, virus, or transgene insertions;
  • monitor established cell lines to rule out interspecies cell culture contamination or other changes during cell culture;
  • ensure the fidelity of embryonic stem cells prior to production of knockout mice;
  • determine the pattern of chromosomal gains and losses; and
  • enumerate copy numbers of chromosomes or cancer-related genes.

Read more about our services.