UPCI's Cell and Tissue Imaging Facility (CTIF) is an integral, cancer-focused component of the University of Pittsburgh's Center for Biologic Imaging. Collaborative efforts between UPCI investigators and CTIF scientists have led to the development of tools specifically directed towards understanding the molecular basis, and perhaps more importantly, potential therapeutic strategies for cancer.
The specific aims of the CTIF are to:
- Provide professional expertise in the design and implementation of experiments using microscopic techniques and image analysis, including interpretation of morphological data.
- Prepare, store, and optimally analyze tissues and cells using morphological, mRNA, and protein localization techniques.
- Provide pathology processing, staining, and review for all projects as needed.
- Evaluate experimental results and propose subsequent experimental direction.
- Provide training in the use of and access to any optical instrument used within the project.
The CTIF is housed in two locations. The first is within the Center for Biologic Imaging, in the Biomedical Science Tower of the University of Pittsburgh School of Medicine. This space was designed as a dedicated, state-of-the-art imaging center, and has fully equipped microscopy suites, computer labs, and wet and dry bench space for cell culture, light and electron microscopic preparations.
The second location is a satellite facility housed within the Hillman Cancer Center. This facility is designed to provide more convenient access to basic confocal, live cell and wide field microscopy to UPCI investigators on the Shadyside campus. This resource is an adjunct to the Oakland resource, which provides access to advanced technologies and electron microscopy.
Learn more about CTIF services.
More than 80% of cancer cells are killed within 6 hours. Movie generated using the Nikon live cell imaging system at the Hillman Cancer Center.