University of Pittsburgh Cancer Institute (UPCI)

CCSG Acknowledgement

Required CCSG Acknowledgement

The NCI requires that publications acknowledge the UPCI CCSG support, and they are tracking compliance. If a UPCI CCSG-supported Shared Resource provided data used in your publication, please include the following statement in the acknowledgment section of your publication(s):

"This project used the UPCI [insert name(s) of shared resource(s)] that [is/are] supported in part by award P30CA047904."

Shared Resource Directors: Please make sure to include this statement on all of your order forms, contracts, etc. as a reminder to your users to acknowledge the UPCI CCSG support.


Overview

UPCI's Cell and Tissue Imaging Facility (CTIF) is an integral, cancer-focused component of the University of Pittsburgh's Center for Biologic Imaging. Collaborative efforts between UPCI investigators and CTIF scientists have led to the development of tools specifically directed towards understanding the molecular basis, and perhaps more importantly, potential therapeutic strategies for cancer.

The specific aims of the CTIF are to:

  • Provide professional expertise in the design and implementation of experiments that use microscopy techniques and image analysis, including interpretation of morphological data.
  • Prepare, store and optimally analyze tissues and cells via morphological and mRNA and protein localization techniques.
  • Provide fixed cell and tissue imaging to UPCI investigators.
  • Provide live cell, live tissue, and live animal imaging to UPCI project leaders.
  • Perform detailed electron microscopy analysis of tissue structure.
  • Provide training related to use of and access to all instrumentation used within the CTIF.
  • Assist with experimental design and refinement, and evaluate experimental results including developing and implementing appropriate software algorithms to provide quantitative measurements.

The CTIF is housed in two locations. The first is within the Center for Biologic Imaging, in the Biomedical Science Tower of the University of Pittsburgh School of Medicine. This space was designed as a dedicated, state-of-the-art imaging center, and has fully equipped microscopy suites, computer labs, and wet and dry bench space for cell culture, light and electron microscopic preparations.

The second location is a satellite facility housed within the Hillman Cancer Center. This facility is designed to provide more convenient access to basic confocal, live cell and wide field microscopy to UPCI investigators on the Shadyside campus. This resource is an adjunct to the Oakland resource, which provides access to advanced technologies and electron microscopy.

Learn more about CTIF services.

Natural killer cells attacking melanoma cells.
More than 80% of cancer cells are killed within 6 hours. Movie generated using the Nikon live cell imaging system at the Hillman Cancer Center.

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