University of Pittsburgh Cancer Institute (UPCI)

Shivendra Singh, PhD

Shivendra Singh
Associate Director for Basic Research, UPCI
UPMC Chair in Cancer Prevention Research
Professor of Pharmacology & Chemical Biology and Professor of Urology, University of Pittsburgh

Contact Information

Contact Information

2.32A Hillman Cancer Research Pavilion
5117 Centre Ave
Pittsburgh, PA 15213

Phone: 412-623-3262
Email: singhs@upmc.edu
Fax: 412-623-7828

Administrative Assistant
Name: Lynnette Robinson
Phone: 412-623-7776
Fax: 412-623-7828

Research Keywords

Research Keywords

Cancer pharmacology; drug discovery; chemoprevention; dietary anti-cancer compounds; isothiocynates; organosulides; withaferin A; cancer prevention

Research Summary

Research Summary


Withaferin A targets beta-tubulin

The primary research interests of the Singh laboratory include molecular characterization of novel cancer chemopreventive agents and rational design of mechanism-driven combination chemoprevention regimens. Cellular and transgenic animal models are used to screen potential cancer chemopreventive constituents from dietary and medicinal plants. Cutting edge cellular, molecular biological, omics (metabolomics and proteomics), structural biology, and imaging techniques (MRI and bioluminescence) are used to (a) determine the mechanism of action of promising cancer chemopreventive agents, (b) monitor effects on cancer progression, and (c) identify biomarkers predictive of tissue exposure and possibly response. Some of the agents under active investigation in the Singh laboratory include: cruciferous vegetable-derived isothiocyanates, garlic-derived organosulides, and medicinal plant constituent withaferin A. As an example, recent published work from the Singh laboratory indicates suppression of glycolysis in mammary cancer prevention by withaferin A in a transgenic mouse model. Likewise, complementary cellular and molecular biological, targeted proteomics, and molecular modeling techniques were used to identify beta-tubulin as a novel target of cancer cell growth arrest by withaferin A (WA).

Selected Publications

Selected Publications

  • Hahm ER, Lee J, Kim SH, Sehrawat A, Arlotti JA, Shiva SS, Bhargava R, Singh SV. Metabolic alterations in mammary cancer prevention by withaferin A in a clinically-relevant mouse model. J Natl Cancer Inst 105: 1111-1122, 2013. PubMed Link
  • Kim SH, Sehrawat A, Singh SV. Dietary chemopreventative benzyl isothiocyanate inhibits breast cancer stem cells in vitro and in vivo. Cancer Prev Res 6: 782-790, 2013. PubMed Link
  • Sehrawat A, Kim SH, Vogt A, Singh SV. Suppression of FOXQ1 in benzyl isothiocyanate-mediated inhibition of epithelial-mesenchymal transition in human breast cancer cells. Carcinogenesis 34: 864-873, 2013. PubMed Link
  • Singh SV, Kim SH, Sehrawat A, Arlotti JA, Hahm ER, Sakao K, Beumer JH, Jankowitz RC, Chandra-Kuntal K, Lee J, Powolny AA, Dhir R. Biomarkers of phenethyl isothiocyanate-mediated mammary cancer chemoprevention in a clinically relevant mouse model. J Natl Cancer Inst 104: 1228-1239, 2012. PubMed Link
  • Powolny AA, Bommareddy A, Hahm ER, Normolle DP, Beumer JH, Nelson JB, Singh SV. Chemopreventative potential of the cruciferous vegetable constituent phenethyl isothiocyanate in a mouse model of prostate cancer. J Natl Cancer Inst 103: 571-584, 2011. PubMed Link

See all pubs (Pubmed)

Collaborations

Collaborations

Current UPCI/Pitt Collaborators

Peter Wipf, PhD
Professor, Department of Chemistry, University of Pittsburgh
Co-Leader - Cancer Therapeutics Program (CTP)

Nathan Yates, PhD
Co-Director, UPCI Cancer Biomarkers Facility; Director, Biomedical Mass Spectrometry Center, University of Pittsburgh School of Medicine
Member - Molecular and Cellular Cancer Biology Program (MCCBP)

Current External Collaborators

Xinhua Ji, PhD
Chief, Biomolecular Structure Section
Center for Cancer Research, National Cancer Institute, Frederick

Adam Marcus, PhD
Associate Professor
Emory University Winship Cancer Institute

Dipali Sharma, PhD
Associate Professor
Johns Hopkins University

UPCI Shared Resource Usage

UPCI Shared Resource Usage

  • Animal Facility
  • Biostatistics Facility
  • Cancer Genomics Facility
  • Cancer Proteomics Facility
  • Cell and Tissue Imaging Facility
  • Cytometry Facility
  • In Vivo Imaging Facility
  • Tissue and Research Pathology Services

Affiliated Graduate Programs

Affiliated Graduate Programs

  • Molecular Pharmacology

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Last modified: 8/1/14