Proteomics Services: Targeted Proteomics
Targeted proteomics is a powerful approach that enables quantitative analysis of pre-specified proteins from complex biological samples with high sensitivity and specificity. Targeted assays can be efficiently developed using in vitro-synthesized proteins and applied to measure proteins in vivo.
The MacCoss laboratory recently described an empirically driven approach for generating targeted protein assays in a scalable, economical and generalizable fashion. The method utilizes the rich collection of tagged cDNA clones that are currently available for most human and model-organism proteins to generate in vitro–synthesized full-length protein samples, followed by tryptic digestion and mass spectrometry analysis using selected reaction monitoring (SRM).
Quantitative proteomics experiments require significant up-front planning and development. Please contact Nadine Ryan to arrange an initial discussion with the proteomics and biostatistics groups. In most cases, custom experiments will be needed to:
- demonstrate that the targeted protein(s) can be detected by mass spectrometry,
- define the amount and type of sample that is required to detect the pre-specified proteins,
- optimize the sample collection methods,
- develop a reproducible sample processing procedures,
- build a mass spectrometry assay, and
- refine the statistical analysis methods that will be used to analyze the data.