University of Pittsburgh Cancer Institute (UPCI)

CCSG Acknowledgement

Required CCSG Acknowledgement

The NCI requires that publications acknowledge the UPCI CCSG support, and they are tracking compliance. If a UPCI CCSG-supported Shared Resource provided data used in your publication, please include the following statement in the acknowledgment section of your publication(s):

"This project used the UPCI [insert name(s) of shared resource(s)] that [is/are] supported in part by award P30CA047904."

Shared Resource Directors: Please make sure to include this statement on all of your order forms, contracts, etc. as a reminder to your users to acknowledge the UPCI CCSG support.


Proteomics Services: Luminex Core Laboratory

The versatile laboratory multianalyte profiling (LabMAP™) system developed by Luminex Corporation, located in Austin, Texas, is a flexible, easy-to-use protein analysis system that permits the simultaneous quantitative analysis of up to 100 different proteins, peptides, or DNA molecules in a single microtiter well. This multiplexing feature allows one to obtain a wealth of information using a minimum amount of sample. Multiplexing enables a comprehensive overview of complex biological systems and the inclusion of in-process controls. In addition, sample volume requirements are reduced and assay throughput is dramatically increased. Potential applications include:

  • functional genomics
  • validation of drug leads
  • secondary screening
  • absorption and distribution
  • metabolism
  • high-throughput ELISA and Western blot assays.

With the Luminex suspension array system you can:

  • Quantitate up to 100 protein targets from culture media, sera, and other matrices simultaneously
  • Automatically analyze up to 96 samples in 60 minutes (general format: 88 sample wells + 8 standard wells)
  • Dramatically increase the amount of useful data

Luminex

Available Analytes

Commercial Kits

Core Developed Assays

Circulating Antigens:
Growth and angiogenic factors: EGFR, angiostatin, endostatin, thrombospondin, IL-18
Cancer Antigens: CA 125, CA 15-3, CEA, AFP, CA 19-9, CA72-4, mammaglobin
Growth factors/Oncogenes: Bcl-2, EGFR, Her2/neu, IGFBP-1, IGFBP-2, SCC
Proteases: PSA, fPSA, Kallikreins 10, Transglutaminase II
Other Markers: CD105, Cyfra 21-1, EPCAM, HE4, mesothelin, MICA, NSE, Ostepontin, TTR, SAA, SMR, Hsp70, VWF

Please contact the facility for specific kit information.

Selected Luminex Partners

Multiplexed Cell Signaling Protein Detection

Several companies offer kits for the multiplex detection for human, non-human primate, canine, porcine, mouse, and rat cytokines. They also offer a wide variety of ancillary and “general-purpose” reagents, including microspheres labeled with capture probes that specifically bind avidin (biotin), immunoglobulins (secondary antibodies and protein A/G), or common fusion protein tags (Ni2+ and glutathione).

Invitrogen
Akt, JNK1/2, p38 3-plex (phospho).
This reagent set comprises the analyte specific components for the measurement of Akt when phosphorylated at serine 473, JNK1/2 when phosphorylated at threonine 183 and tyrosine 185, and p38 MAPK when phosphorylated at threonine 180 and tyrosine 182 in cell lysates/tissue homogenates.

Multiplexed Transcription Factors Technology

Multiplexed Transcription Factors Technology

Multiplexed Transcription Factor Profiling System allows researchers to identify proteins that drive biological responses. It employs a powerful multiplexing approach for simultaneously measuring the binding of many different transcription factors to their unique regulatory DNA elements. The technology dramatically accelerates studies ranging from basic research in signal transduction and proteomics to target validation, compound screening, toxicity testing and disease characterization. This innovative technology offers unique benefits to researchers studying gene regulation. Unlike conventional methods such as EMSAs, each assay measures the presence of multiple transcription factors simultaneously, allowing powerful comparisons of different cell lines and treatments. The technology utilizes a strategy similar to nuclease protection assays. A schematic diagram of the technology is to the right.

QuantiGene® Plex Reagent System (Affymetrix)

QuantiGene® Plex Reagent System is Affymetrix's (formerly Panomics and Genospecta) proprietary cell-based system for multiplexed gene expression measurements in RNAi, predictive toxicology and drug development. QuantiGene Plex is the perfect marriage of two groundbreaking technologies – xMAP® (multi-analyte profiling beads from Luminex Corp) and bDNA (branched DNA signal amplification technology). Beyond the benefits of QuantiGene, this system allows one to quantitate multiple mRNA targets simultaneously – directly from a cell lysate or tissue homogenate – without the need for RNA purification, reverse transcription, or amplification. Quantigene Plex products have the same excellent accuracy, precision, and speed as the rest of the QuantiGene line.

More information on the QuantiGene Reagent System is available here.