University of Pittsburgh Cancer Institute (UPCI)

CCSG Acknowledgement

Required CCSG Acknowledgement

The NCI requires that publications acknowledge the UPCI CCSG support, and they are tracking compliance. If a UPCI CCSG-supported Shared Resource provided data used in your publication, please include the following statement in the acknowledgment section of your publication(s):

"This project used the UPCI [insert name(s) of shared resource(s)] that [is/are] supported in part by award P30CA047904."

Shared Resource Directors: Please make sure to include this statement on all of your order forms, contracts, etc. as a reminder to your users to acknowledge the UPCI CCSG support.


Fee Structure

For more information regarding pricing for mass spectrometry-based proteomics projects, contact Dr. Nathan Yates.

To find out more about sequencing pricing, please contact Maureen Lyons or Patricia Petrosko.

Extraction and QA/QC Services

Extractions- Fresh and Frozen Tissue, FFPET, Cell Culture, Fluids

All extractions require QC via Bioanalyzer and UV Spectrophotometry via Nanodrop

RNA USD
RNA Extraction Qiagen RNeasy Midi $116.00
RNA Extraction Qiagen RNeasy Mini $108.00
RNA Extraction Qiagen RNeasy Micro $107.00
RNA Extraction Qiagen miRNeasy $104.00
DNA
DNA Extraction Qiagen $83.00
DNA Extraction Whole Blood- Flexigene $70.00
Custom Paraffin Sample Extraction- 10um Slides or Scrolls
RNA Extraction from FFPET Samples- Qiagen RNeasy FFPET - Scrolls $110.00
RNA Extraction from FFPET Samples- Qiagen RNeasy FFPET - Slides $130.00
RNA Extraction from FFPET Samples- Qiagen MiRNeasy FFPET $110.00
RNA Extraction from FFPET Samples- RecoverAll from Scrolls $135.00
DNA Extraction Qiagen QIAamp (includes deparaffinization) $82.00
Macrodissection from FFPET slides (does not include deparaffinization or extraction) $38.00
Nucleic Acid Quality Analysis
Nucleic Acid Quantitation via nanodrop (DNA or RNA) $3.00
Nucleic Acid Quantitation (DNA or RNA)- QuBit $5.00
Bioanalyzer Services- Prices per chip, up to 12 samples
RNA Quality Analysis- Nano Chip $73.00
RNA Quality Analysis- Pico Chip $76.50
FFPET DNA Quality Analysis- 7500 Chip $75.00


Genotyping Services: Affymetrix

Genotyping Service- Affymetrix Array Assays

6.0 Assay
DNA Genotyping 6.0 Assay (Labeling, Hybridization, Scan) $436.00 per sample
Affymetrix Human Mapping 6.0 Array $342.00 per sample
Total Cost per Sample for Affymetrix Genotyping 6.0 Assay $778.00
500K Assay
DNA Genotyping 500K Assay (250K Sty Assay + 250K NSP Assay) $600.00 per sample
Affymetrix Human Mapping 250K Nsp Array $171.00 per sample
Affymetrix Human Mapping 250K Sty Array $171.00 per sample
Total Cost per Sample for Affymetrix Genotyping 500K Assay $942.00
250K Assay
DNA Genotyping (250K Sty Assay) $300.00 per sample
Affymetrix Human Mapping 250K Sty Array $171.00 per sample
Total Cost per Sample for Affymetrix Genotyping 250K STY Assay $471.00
DNA Genotyping (250K NSP Assay) $300.00 per sample
Affymetrix Human Mapping 250K NSP Array $171.00 per sample
Total Cost per Sample for Affymetrix Genotyping 250K STY Assay $471.00


Genotyping Services: TaqMan Assays


Genotyping Service – TaqMan Assays
TaqMan Genotyping Assay $580 per plate
TaqMan Genotyping Assay Plate Read Only $25 per plate
TaqMan Copy Number Variation Assays $950 per plate


Tiling Microarray Assay


Affymetric Promoter Tiling Assay*
* Researcher performs ChIP assay and provides CGF dbl stranded, IP'd DNA- de-crosslink'd and prot k treated
DNA Promoter Array Assay* $421.00 per sample
Affymetrix Human Promoter 1.0R Array $375 per sample
Total cost per sample for Affymetrix Genotyping 250K STY Assay $796


Expression Services: Affymetrix

Expression Service- Affymetrix 3' Array Assay-

For 3' Arrays (Affy HGU133 Plus 2.0, HGU133A 2.0, etc)

Ambion Labeling Assay
Ambion MessageAmp Premier Whole Assay (Labeling, Hybridization, Scan) $372.00 per sample
Affymetrix U133 Plus 2.0 Array (Whole Genome) $456.00 per sample
Total Cost per Sample for Affymetrix HGU133 Plus 2.0 Assay $810.00
Ambion MessageAmp Premier Whole Assay (Labeling, Hybridization, Scan) $354.00 per sample
Affymetrix U133A 2.0 Array $285.00 per sample
Total Cost per Sample for Affymetrix HGU133A 2.0 Assay $639.00
Affymetrix SensationPlus FFPET Assay
Affymetrix SensationPlus FFPET Whole Assay (Labeling, Hybridization, Scan) $483.00 per sample
Affymetrix U133 Plus 2.0 Array (Whole Genome) $456.00 per sample
Total Cost per Sample for Affymetrix HGU133 Plus 2.0 Assay $939.00
Affymetrix SensationPlus FFPET Whole Assay (Labeling, Hybridization, Scan) $483.00 per sample
Affymetrix U133A 2.0 Array $285.00 per sample
Total Cost per Sample for Affymetrix HGU133 2.0 Assay $768.00
NuGen Ovation Assay- For samples with low starting input (500pg-50ng)
NuGen Ovation Pico Assay (Labeling, Hybridization, Scan) $442.00 per sample
Affymetrix U133 Plus 2.0 Array (Whole Genome) $456.00 per sample
Total Cost per Sample for Affymetrix HGU133 Plus 2.0 Assay $898.00
NuGen Ovation Pico Assay (Labeling, Hybridization, Scan) $442.00 per sample
Affymetrix U133A Plus 2.0 Array (Whole Genome) $456.00 per sample
Total Cost per Sample for Affymetrix HGU133A Plus 2.0 Assay $898.00
NuGen Ovation Pico Assay (Labeling, Hybridization, Scan) $442.00 per sample
Affymetrix U133A 2.0 Array $285.00 per sample
Total Cost per Sample for Affymetrix HGU133A 2.0 Assay $727.00
NuGen FFPET Assay- For FFPET Samples
NuGen Ovation FFPET Assay (Labeling, Hybridization, Scan) $406.00 per sample
Affymetrix U133 Plus 2.0 Array (Whole Genome) $456.00 per sample
Total Cost per Sample for Affymetrix HGU133 Plus 2.0 Assay $1,024.00
NuGen Ovation FFPET Assay (Labeling, Hybridization, Scan) $568.00 per sample
Affymetrix U133A 2.0 Array $285.00 per sample
Total Cost per Sample for Affymetrix HGU133A 2.0 Assay $853.00

Expression Service- Affymetrix Whole Transcript Array Assays

WT Expression Assays for Affy Gene ST and Exon ST Arrays

Ambion WT Expression Labeling Assay
Ambion WT Expression Assay (Labeling, Hybridization, Scan) $393.00 per sample
Affymetrix Human Exon 1.0 ST Array $456.00 per sample
Total Cost per Sample for Affymetrix Exon 1.0 ST Assay $849.00
Ambion WT Expression Assay (Labeling, Hybridization, Scan) $393.00 per sample
Affymetrix Human Gene 1.0 ST Array $200.00 per sample
Total Cost per Sample for Affymetrix Gene 1.0 ST Assay $593.00
NuGen WT Expression Assay
NuGen WT Expression Assay (Labeling, Hybridization, Scan) $332.00 per sample
Affymetrix Human Exon 1.0 ST Array $456.00 per sample
Total Cost per Sample for Affymetrix Exon 1.0 ST Assay $788.00
NuGen WT Expression Assay (Labeling, Hybridization, Scan) $332.00 per sample
Affymetrix Human Gene 1.0 ST Array $200.00 per sample
Total Cost per Sample for Affymetrix Gene 1.0 ST Assay $532.00
NuGen WT Expression Assay- FFPET Samples
NuGen WT Expression Assay- FFPET Samples $573.00 per sample
Affymetrix Human Exon 1.0 ST Array $456.00 per sample
Total Cost per Sample for Affymetrix Exon 1.0 ST Assay $1,029.00
NuGen WT Expression Assay- FFPET Samples $573.00 per sample
Affymetrix Human Gene 1.0 ST Array $200.00 per sample
Total Cost per Sample for Affymetrix Gene 1.0 ST Assay $773.00


Expression Services: CodeLink Array Assay


Ambion MessageAmp Premier Whole Assay (Labeling, Hybridization, Scan) $454.00 per sample
CodeLink Whole Genome Array (Human, Mouse, Rat) $143.00 per sample
Total Cost per Sample for CodeLink Assay $597.00


miRNA Expression Services: Affymetrix miRNA Array Assay


Affymetrix Whole Assay (Labeling, Hybridization, Scan) $336.00 per sample
Affymetrix miRNA 3.0 Array $302.00 per sample
Total Cost per Sample for Affymetrix miRNA Assay $638.00


miRNA Expression Services: Exiqon miRNA Array Assay


Exiqon Whole Assay (Labeling, Hybridization, Scan) $240.00 per sample
Exiqon miRCURY miRNA Array $445.00 per sample
Total Cost per Sample for Exiqon Assay $685.00


Expression Services: TaqMan Assay


TaqMan Assay
TaqMan Expression Assay (Set up and Plate Run) $33.00 per sample
TaqMan Expression Plate Run Only $100.00 per plate
TaqMan miRNA Assay
miRNA TLDA v3.0 (Array A and B- Set up and Plate Run) $845.00 per array
miRNA Single Target TaqMan Assay (Set up only) $33.00 per sample
miRNA TLDA Plate Run Only $100.00 per plate
Taqman miRNA Plate Run Only $100.00 per plate


SearchLight Proteomics Assays ***


Custom SearchLight
1 – 40 samples in duplicate; must order at least 5 kits or price will increase accordingly
1 plex $932.04
2 plex $965.04
3 plex 1,075.04
4 plex $1,282.94
5 plex $1,440.24
6 plex $1,561.24
SearchLight Commercial Kits, Human (1 - 20 samples)
BioMarker Array 1: Apo A-1, CRP, ACRP-30 $1,185.82
Vascular Injury Array: E-Selectin, ICAM-3, P-Selectin, Thrombomodulin $1,273.32
Vascular Injury Array: ICAM-1, SAA, VCAM-1 $1,185.82
Angiogenesis Array:FGF, HGF, PDGF-BB, VEGF $1,273.32
Angiogenesis Array:HB-EGF, KGF, PDGF-AA, Tpo, VEGFR-1, VEGFR-2 $1,585.82
Cytokine Array:IFNg, IL-1a, IL-1b, IL-2, Il-6, IL-8, IL-10, IL-12p70, TNFa $2,023.32
Cytokine Array:IFNg, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70, IL-13, TNFa $2,023.32
Cytokine Array:IFNg, IL-1b, IL-6, TNFa $1,273.32
Chemokine Array:Eotaxin, I-309, IL-8, MCP-1, MDC, MIP-1a, MIP-1b, RANTES, TARC $2,023.32
Chemokine Array:ENA-78, GRO alpha, IL-8, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, NAP-2 $2.023.32
MMP Array:MMP1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2 $2,023.32
SearchLight Commercial Kits, Rat (1 - 20 samples)
GM-CSF, IFNg, IL-1a, IL-1b, IL-2, IL-4, IL-6, IL-10, TNFa $2,109.90
Fractalkine, KC/GRO alpha, MCP-1, MIP-1a, MIP-2, MIP-3a, RANTES $1,849.90

*** For more commercially available SearchLight assays, please visit the Aushon website and inquire.


Other Services

Custom Array Scanning (Axon 4000B Scanner) $65.00
Data Analysis (per hour rate) $78.00
Training (per hour rate) $78.00
Equipment Useage (per hour rate) $78.00


Luminex

All LUMINEX Instrument Analysis Performed By the Facility Operators $144.99/plate
Plate processing with commercially available kits. The customer provides the samples and kit(s) purchased either directly from one of the manufacturers or through the core. Several companies have extended discounted pricing which we can pass along to our customers. The Luminex Core Laboratory processes the plate(s) and provides the data as an Excel spreadsheet. $374.99
Plate processing with kits developed in the Luminex Core Laboratory. The customer provides the samples in 96-well plate format. The Luminex Core Laboratory processes the plate(s) using an in-house kit and provides the data as an Excel spreadsheet. $409.99 + $200/analyte
Kit development by Luminex Core Laboratory: The customer provides the antibodies (monoclonal for capture, polyclonal for detection) and protein standard. Luminex personnel are available for consultation and the assay development is offered on a no-guarantee basis. $630/$630/$840 = $2,100.00
Additional Charges
Data normalization and/or consultation $20/hour
($10 minimum)
Virus Cleanup $50
Equipment Rental - Vacuum Manifold/Shaker/Instrument Fee $275
Per Column Service (7-pt curve + Blank + 1 Column Samples)
Human 39-Plex (Millipore)
IL-1a, IL-1b, IL-ra, IL-2, sIL-2Ra, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17a, sCD40L, EGF, Eotaxin, FGF-2, Flt-3L, Fractalkine, G-CSF, GM-CSF, GRO, IFNa2, IFNg, IP-10, MCP-1, MCP-3/CCL7, MDC/CCL22, MIP-1a, MIP-1b, TGFa, TNFa, TNFb, VEGF
$1,111.67
Human 30-Plex (Invitrogen)
EGF, Eotaxin, FGF-b, G-CSF, GM-CSF, HGF, IFN-a, IFN-g, IL-1b, IL-1Ra, IL-2, IL-2R, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12(p40/p70), IL-13, IL-15, IL-17, IP-10, MCP-1, MIG, MIP-1a, MIP-1b, RANTES, TNF-a, VEGF
$685.12
Human 27-Plex (Bio-Rad)
IL-1b, IL-1Ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12(p70), IL-13, IL-15, IL-17, Eotaxin, FGF-b, G-CSF, GM-CSF, IFN-g, IP-10, MCP-1, MIP-1a, MIP-1b, PDGF-BB, RANTES, TNF-a, VEGF
$881.17
Human 23-Plex (Bio-Rad)
IL-1a, IL-2Ra, IL-3, IL-12(p40), IL-16, IL-18, CTACK, GRO-a, HGF, ICAM-1, IFN-a2, LIF, MCP-3, M-CSF, MIF, MIG, b-NGF, SCF, SCGF-b, SDF-1a, TNF-b, TRAIL, VCAM-1
$758.33
Mouse 32-Plex (Millipore)
IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17a, EOTAXIN, G-CSF, GM-CSF, INF-g, , IP-10, KC, LIF, LIX, M-CSF, MCP-1, MIG, MIP-1a, MIP-1b, MIP-2, RANTES, TNFa, VEGF
$986.67
Mouse 20-Plex (Invitrogen)
FGF-b, GM-CSF, IFN-g, IL-1a, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p40/p70), IL-13, IL-17, IP-10, KC, MCP-1, MIG, MIP-1a, TNF-a, VEGF
$554.50
Other Assay Fees
Molecular Biology Assays (TF's Phosphos, etc.) $469.99
Protein Determination/Sample Dilution Fee $49.99 per 1-20 samples