University of Pittsburgh Cancer Institute (UPCI)

Services

The BMF uses citable, validated assay methodologies refined and standardized in the laboratory to provide rapid and reliable results of high specificity and sensitivity with blood, saliva, and urine samples.

Our services are available to investigators at UPCI as well as investigators at any nonprofit laboratory/institution in the United States by arrangement with the Facility Director.

Assay List

  • Catecholamines* (epinepherine, norepinephrine, and dopamine) in plasma, urine and platelets by HPLC
  • Cytokines, chemokines, viral antibodies, and 8-OHDG in serum and plasma by ELISA
  • Cortisol in saliva, plasma, and urine by ELISA
  • Creatinine and electrolytes (Na+, K+) in urine**
  • Cotinine, 3-OH cotinine, and nicotine in urine
  • Natural Killer Cell cytotoxicity assays (whole blood or lymphocyte)
  • Comet assay (DNA damage assay)
  • New assay development available upon request

*Catecholamines are widely used in biobehavioral studies as independent measures of acute and/or chronic stress. All catecholamine assays are performed in duplicate using a high pressure liquid chromatography (HPLC) and electrochemical detector from ESA (Chelmsford, MA).

Catecholamine levels in urine or platelet collection reflect the average levels over a period of time, while levels in plasma reflect short-term or acute levels.

**Creatinine is commonly used as an internal control for the amount of urine in each sample.